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Oligonucleotides used for site-directed mutagenesis
The underlined bases correspond to the genetic codes for the amino acid(s) to be mutated.
| Primer | Sequence |
|---|---|
| R94A | |
| Forward | GCTTCATGCTGGCCTACCTGCATGCG |
| Reverse | CGCATGCAGGTAGGCCAGCATGAAGC |
| R94N | |
| Forward | GCTTCATGCTGAACTACCTGCATGCG |
| Reverse | CGCATGCAGGTAGTTCAGCATGAAGC |
| R94D | |
| Forward | GCTTCATGCTGGACTACCTGCATGCG |
| Reverse | CGCATGCAGGTAGTCCAGCATGAAGC |
