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Characterization of mutants
The cytochrome bc1 complexes were in 50 mm Tris-Cl (pH 8.0) containing 200 mm NaCl, 200 mm histidine, 0.5% octyl glucoside, and 10% glycerol.
| Strain | Photosynthetic growth | Activitya |
|---|---|---|
| WT | ++++ | 4.58 (100%) |
| R94A | + | 0.18 (4%) |
| R94D | ++ | 1.61 (35%) |
| R94N | +++ | 2.34 (51%) |
a The enzyme-specific electron transfer activity of the purified bc1 complexes is expressed as micromoles of cytochrome c reduced per min/nmol of cytochrome b at room temperature.
