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. 2012 Nov 27;288(2):1055–1064. doi: 10.1074/jbc.M112.423384

FIGURE 3.

FIGURE 3.

Slowing the RecB motor (RecBY803H) slows both the 3′ to 5′ and 5′ to 3′ translocation rates, whereas slowing the RecD motor (RecDY567H) slows only the 5′ to 3′ translocation rate. A, monitoring 3′ to 5′ ssDNA translocation of RecBY803HCD using DNA I. Inset, rate = 441 ± 13 nt/s. B, monitoring 5′ to 3′ ssDNA translocation of RecBY803HCD using DNA II. Inset, rate = 1454 ± 75 nt/s. C, monitoring 3′ to 5′ ssDNA translocation of RecBCDY567H using DNA I. Inset, rate = 1412 ± 12 nt/s. D, monitoring 5′ to 3′ ssDNA translocation of RecBCDY567H using DNA II. Inset, rate = 1046 ± 108 nt/s. AU, arbitrary units.