FIGURE 2.

Inhibition of mitochondrial JNK signaling and JNK activity prevents 6-OHDA-induced oxidative stress in SHSY5Y cells. A, SHSY5Y cells were treated with 25 μm 6-OHDA for 18 h, and normalized reduced glutathione levels were measured in the presence or absence of 500 nm SR-3306, 10 μm Tat-Sab, or Tat-scramble peptide, JNK siRNA, Sab siRNA, or control siRNA. B, same as in A except LPO was measured. C, same as in A except protein carbonyls were measured. Significance (p < 0.05) between 6-OHDA-treated and -untreated groups is shown by * and between 500 nm SR-3306, 10 μm Tat-Sab or JNK siRNA, or Sab siRNA- and 6-OHDA-treated groups is given by **. The results are from at least three biological replicate experiments, each performed in quadruplicate.