FIGURE 3.

The multiligand receptors LRP2 and cubilin colocalize at the apical surface of the endodermal epithelial cells of the yolk sac. Cryosections (4 μm) of the area vasculosa of yolk sac tissue from 10-day-old chicken embryos were prepared as described under “Experimental Procedures.” The sections were stained with our rabbit anti-ggLRP2 antiserum (green staining) and our mouse anti-ggCubilin antiserum (red staining). The merged image is shown on the right. For the visualization of cell nuclei, the sections were counterstained with DAPI. Images were acquired using a Zeiss LSM 510 confocal microscope. Scale bar, 50 μm.