Effect of heparin, HS and K5NOSH on monocyte migration induced by p17. Monocytes in the absence (control, ctrl) or in the presence of heparin, HS and K5NOSH (all at 1 and 10 μg/ml) were added to the top well, whereas medium alone or containing p17 (59 nm) or fMLP (10 nm) was added to the bottom well. Bars represent the mean ± S.D. of three independent experiments performed in duplicate. Statistical analysis was performed using GraphPad Prism software (version 5, GraphPad Software, Inc.) and one-way analysis of varaince and Bonferroni's post test was used to compare data. *, p < 0.01 statistically different compared with p17-stimulated cells. NT, not treated cells.