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. 2001 Jul 3;98(14):7846–7851. doi: 10.1073/pnas.141124698

Figure 2.

Figure 2

(A) T84 cells were synchronized by starvation in EMEM depleted of l-glutamine for 24 h. Proliferation was stimulated with EMEM containing 10 mM l-glutamine; cells were incubated for 48 h with 1 μM ST or PBS, and quantified as described in Fig. 1A. Values are expressed as the percentage increase in cell number stimulated by l-glutamine relative to those values at t0. Total cells: at t0 = 0.4 × 106 ± 0.07; at 48 h, control = 1.4 × 106 ± 0.2; ST = 0.9 × 106 ± 0.06. (B) Cells were synchronized and stimulated to proliferate by glutamine (as described in A), and [3H]thymidine and 1 μM ST (■) or PBS (□) was added and incubated for the indicated times. At the conclusion of incubations, [3H]thymidine incorporation into DNA was quantified as described in Materials and Methods. Values reflect a representative experiment. In B2, data obtained in B1 are expressed as {100 − [([3H]thymidine incorporation in ST-treated incubations)/([3H]thymidine incorporation in control incubations) × 100]}. *, P < 0.05; **, P < 0.01.