FIGURE 2.
ELMO1 and ACF7 cooperate to promote the formation of membrane protrusions. A–D, CHO LR73 cells transfected with: HA-ACF7 (A), Myc-ELMO1 (B), Myc-ELMO1 and HA-ACF7 (C), and Myc-ELMO1PxxP and HA-ACF7 (D). Following serum starvation, the cells were plated on fibronectin-coated chambers for 45 min. The cells were stained for ELMO1 (anti-ELMO1) and ACF7 (anti-HA). The arrows indicate membrane protrusions. Scale bar, 10 μm. E, quantification of the effects of the ELMO1-ACF7 interaction on cell elongation. CHO LR73 cells were prepared as in A–D. For each condition, the Feret's diameter of >40 cells were measured. ANOVA tests and Bonferroni's multiple comparison were performed to compare each condition (***, p < 0.001; error bars represent 10–90 percentiles, n = 3).