FIGURE 5.

Non-Mendelian inheritance of chaperone gene deletion-induced changes in HA-Rnq1p aggregate size. Diploids generated by mating chaperone deletion stains with a wild-type [psi⁻][pin⁻] strain were sporulated and dissected. The resulting tetrads were analyzed for HA-Rnq1p aggregate size by SDD-AGE. Wild-type strains were mated with hsp104Δ strains to demonstrate genetic specificity for this assay. Wild-type (+) and deletion-carrying (−) haploid progeny are presented. The hsp104Δ samples and wild-type controls were run on a continuous gel and exposed for a longer period. Twenty-four tetrads were analyzed per diploid.