TABLE 1.

Protein spot analysis and LC-MS/MS identification

Spot	% of displacementa	Protein ID	NCBI accession number	Theoreticalb molecular mass	Observedc molecular mass	Theoreticalb pI	Observedc pI	% of sequence coverage	Spectra count
				Da	Da
4	40.1	VDAC-2	gi 62826006	30,183	27,937	8.36	8.99	29	18
6	52.0	VDAC-2	gi 62826006	30,183	27,448	8.36	8.27	23	14
12	46.5	VDAC-1	gi 28302268	30,627	28,671	6.85	6.71	20	11
22	74.5	VDAC-1	gi 28302268	30,627	29,160	6.85	6.21	26	16
33	3.4	SNAP-25	gi 33416802	23,172	26,468	4.74	4.89	30	15
41	1.7	Gβ4	gi 49257618	37,504	33,084	5.70	5.78	20	11
85	26.6	PDIA3	gi 28302197	56,086	54,992	5.72	5.91	30	25
	26.6	VHA-55	gi 28436920	56,411	54,992	5.56	5.91	20	21

^a [³H]AziPm displacement by propofol from in vitro photolabeling experiments.

^b Theoretical values were computed with the ExPASy Compute pI/Mw tool. Monoisotopic molecular weights are shown.

^c Observed values were estimated from molecular weight markers and IEF-resolving estimations published by the manufacturer of the gels.