FIGURE 10.

RUNX1 as well as p53 interacts with p300 histone acetyltransferase. A, p300 knockdown. HCT116 cells were transiently transfected with control siRNA or with siRNA against p300. Twenty four hours after transfection, cells were treated with ADR (at a final concentration of 0.5 μm) or left untreated. Twenty four hours after ADR exposure, cell lysates were prepared and analyzed by immunoblotting with the indicated antibodies. w/o, without. B and C, interaction between RUNX1 and p300. HCT116 cells were exposed to 0.5 μm ADR (B) or left untreated (C). After 48 h, cell lysates were prepared from ADR-treated and -untreated cells, and immunoprecipitated (IP) with the indicated antibodies followed by immunoblotting with the indicated antibodies. 1/20 of inputs are also shown (right panels). D, complex formation of p53 with p300 is dependent on RUNX1. HCT116 cells were transiently transfected with control siRNA or with siRNA against RUNX1. Twenty four hours after transfection, cells were treated with 0.5 μm ADR. Twenty four hours after ADR treatment, cell lysates were prepared and immunoprecipitated with anti-p300 antibody. The immunoprecipitates were analyzed by immunoblotting with anti-p53 antibody. 1/20 of inputs are also shown (right panels).