FIGURE 4.

Complex formation between RUNX1 and p53. A, exogenous interaction of RUNX1 with p53. HCT116 cells were transiently transfected with the indicated combinations of the expression plasmids. Forty eight hours after transfection, cell lysates were prepared and immunoprecipitated (IP) with NMS or with anti-p53 antibody. The immunoprecipitates were analyzed by immunoblotting with anti-RUNX1 antibody. The reciprocal experiments using NRS and anti-RUNX1 antibodies are also shown. WB, Western blot. B, endogenous interaction of RUNX1 with p53. HCT116 cells were treated with 0.5 μm ADR. Twenty four hours after ADR treatment, cell lysates were prepared and immunoprecipitated with NMS or with anti-p53 antibody, followed by immunoblotting with anti-RUNX1 antibody. The reciprocal experiments are also shown.