FIGURE 7.

Knocking down RUNX1 suppresses ADR-mediated acetylation and transcriptional activity of p53. HCT116 cells were transiently transfected with control siRNA or with siRNA against RUNX1. Twenty four hours after transfection, cells were incubated in the presence or absence of ADR (at a final concentration of 0.5 μm). Twenty four hours after ADR exposure, cell lysates and total RNA were extracted and processed for immunoblotting (upper panels) and RT-PCR (lower panels), respectively. w/o, without.