FIGURE 8.

Knocking down RUNX1 suppresses ADR-mediated apoptosis in HCT116 cells. A, phase-contrast micrograph. HCT116 cells were transiently transfected with control siRNA or with siRNA targeting RUNX1 for 24 h, after which cells were exposed to ADR (at a final concentration of 1 μm) or left untreated. Twenty four hours after ADR treatment, cells were examined by phase-contrast microscope. B, trypan blue exclusion assay. RUNX1 knocked down HCT116 cells were exposed to 1 μm ADR or left untreated. Twenty four hours after ADR treatment, floating and attached cells were collected and stained with 0.4% trypan blue. After trypan blue staining, the number of trypan blue-positive cells was measured.