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. 2012 Nov 12;288(2):1365–1373. doi: 10.1074/jbc.M112.434142

FIGURE 2.

FIGURE 2.

HMGB1 inhibits VEGF-induced HPAEC migration. A, representative images of cell migration assay of HPAECs treated for 10 h with VEGF (50 ng/ml) or VEGF + HMGB1 (1 μg/ml). B, serum-starved HPAECs stimulated with VEGF (50 ng/ml) in the absence/presence of HMGB1 (100 ng/ml–1 μg/ml) and assessed for cellular migration using the monolayer wound assay. C and D, effect of HMGB1 (1 μg/ml) on VEGF (50 ng/ml)-stimulated HUVEC migration (C) and HDMEC migration (D). E, effect of polymyxin on HMGB1-inhibited HPAEC migration (1000 ng/ml). F, effect of HMGB1 on HPAEC proliferation using [3H]thymidine incorporation. Data represent the mean ± S.E. (error bars) of three independent experiments. Analysis of variance; *, p < 0.05.