FIGURE 3.

TLR4 mediates the effects of HMGB1 of HPAEC migration. A, quantitative PCR for TLR4 in control and TLR4 siRNA-treated HPAECs. B, HPAECs were transfected with TLR4 siRNA (25 nm) or control siRNA (25 nm) and VEGF-induced cell migration assessed in the presence/absence of HMGB1 (1 μg/ml). (C) HPAECs were treated with TLR4 blocking antibody (20 μg/ml) or control IgG (20 μg/ml) and VEGF-induced cell migration assessed in the presence/absence of HMGB1 (1 μg/ml). Data represent the mean ± S.E. (error bars) of three independent experiments. Analysis of variance; *, p < 0.05.