FIGURE 9.

Inhibition of HPAEC migration by HMGB1 is IRF3-dependent. A, relative MyD88 expression assessed by quantitative RT-PCR after treating HPAECs with control or MyD88siRNA. B, VEGF-stimulated cell migration assessed in HPAECs treated with siRNA against MyD88 (25 nm) or with control siRNA (25 nm) in the presence/absence of HMGB1 (1 μg/ml). C, relative IRF-3 expression using quantitative PCR after treating HPAECs with scramble or IRF-3 siRNA. D, VEGF-stimulated cell migration assessed in HPAECs treated with siRNA against IRF3 (25 nm) or with control siRNA (25 nm) in the presence/absence of HMGB1 (1 μg/ml). In all panels, data represent the mean ± S.E. (error bars) of three independent experiments. Analysis of variance; *, p < 0.05.