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Characterization of the peptide-antibody fusion G6-LC in serum. A, SDS-PAGE of G6-LC peptide-antibody fusion protein following incubation in mouse serum for 48 h. The IL17A peptide was cleaved nonspecifically at numerous positions. B, mass spectroscopic extracted ion chromatograms of the intact anti-IL17A peptide CVX5484 of the peptide-antibody fusion G6-LC and one example of the detected truncations, as described under “Experimental Procedures.”
