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The removal of an unexpected O-linked glycosylation modification is crucial to retaining the full anti-IL17A activity of the peptide-antibody scaffold. A, kinetic analysis with human IL17A. B, IL17A inhibition curves for the peptide-antibody fusion with either the original sequence (Q-GSE21-LC) or the threonine mutation to alanine (Q-GSE21-LC-T5A). Data shown are from a single representative experiment.
