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. 2012 Nov 27;288(2):793–806. doi: 10.1074/jbc.M112.413716

FIGURE 5.

FIGURE 5.

Rad14p associates with the promoters and coding sequences of the GAL7 and GAL10 genes in a transcription-dependent manner. A, the schematic diagrams show the PCR primer pairs located at the promoters and ORFs of the GAL7 and GAL10 genes in the ChIP assay. The numbers are presented with respect to the position of the first nucleotide of the initiation codon (+1). B and C, Rad14p associates with the promoters and coding sequences of the active GAL7 and GAL10 genes. The yeast strain expressing Myc epitope-tagged Rad14p (ZDY3) was grown, cross-linked, and immunoprecipitated as in Fig. 1B. D, RT-PCR analysis is shown. Both the wild type and Δrad14 strains (ZDY2 and PCY25) were grown for analysis of GAL7 and GAL10 transcriptions as in Fig. 1F. E and F, analysis of Rpb1p association with the promoters and coding sequences of the GAL7 and GAL10 genes in the Δrad14 strain (PCY25) and its isogenic wild type equivalent. The wild type and Δrad14 strains were grown, cross-linked, and immunoprecipitated as in Fig. 2A.