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Knockdown of DUSP14 enhances phosphorylation of TAK1 at Thr-187. The 293 cells were transfected with control or DUSP14 RNAi plasmid for 48 h. The cells were either left untreated or treated with TNF (10 ng/ml; A) or IL-1 (10 ng/ml; B) for the indicated times. Cell lysates were analyzed by immunoblotting with anti-phospho-TAK1 (Thr-187), anti-TAK1, anti-DUSP14, and anti-β-actin antibodies.
