FIGURE 7.

Somatic mutations in the Ig-Sμ region and the c-myc gene of MEFs after treatment with H₂O₂. Bcl6-KO and WT MEFs at P5 were treated with various doses of H₂O₂ for 2 h and then cultured for 96 h. A, cell numbers of WT (open bar) and Bcl6-KO (filled bar) MEFs were calculated 24 and 96 h after treatment. Data are presented as a representative of three independent experiments. B, amounts of ADAR1 mRNA in MEFs after treatment with 100 μm H₂O₂ were analyzed by real-time qRT-PCR. Data are presented as a representative of three independent experiments. C, pie charts indicate mutations in the Ig-Sμ region and the c-myc gene of MEFs after treatment with 100 μm H₂O₂. Sectors of the pie are proportional to the number of sequences with a given number of mutations. The number of sequences is shown in the center of the pie chart. A frequency of mutations is described below the pie chart.