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. 2012 Nov 19;288(2):886–893. doi: 10.1074/jbc.M112.417089

FIGURE 3.

FIGURE 3.

The expression of Stil in developing embryos and adult zebrafish. A, qRT-PCR analysis of Stil expression at different ages. The lowest expression in 3-month-old fish was normalized to 1. The highest expression was detected in developing embryos (at 1 day post-fertilization). Data represent the means ± S.E. (n > 30 in embryos; n = 9 in each juvenile and adult stage). B, in situ hybridization against Stil mRNA at 1 day post-fertilization. Stil was highly expressed in the brain and spinal cord. Scale bar, 100 μm. C), qRT-PCR analysis of Stil expression in different tissues and organs isolated from adult zebrafish (6 months old). The lowest expression in the heart was normalized to 1. Data represent the means ± S.E. (n = 9). D, single-cell RT-PCR analysis of Stil expression in isolated DA-IPCs (DA), Müller glia cells (M), and photoreceptor cells (P). TH primers were used to confirm the DA cell type.