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. 2012 Nov 19;288(2):886–893. doi: 10.1074/jbc.M112.417089

FIGURE 6.

FIGURE 6.

Effects of 6-OHDA on DA-IPCs in wild-type retinas treated with Shh signaling antagonist cyclopamine. A, RT-PCR analysis of Gli1 expression in control and cyclopamine-treated retinas. The injection of cyclopamine resulted in decreased Shh-targeted Gli1 gene expression. Data represent the means ± S.E. (n = 3 in each group). *, p < 0.01. B, number of anti-TH positive DA-IPCs in control, 6-OHDA-treated, and cyclopamine (Cycl)- and 6-OHDA-co-treated wild-type and heterozygous mutant retinas. Note the decrease in the number of DA-IPCs in co-treated retinas. Data represent the means ± S.E. (n = 9 in each group). ns, not significant; *, p < 0.01. C, fluorescent images of flat-mount control and treated retinas labeled with anti-TH antibodies. Note the decreased number of DA-IPCs in cyclopamine and 6-OHDA co-treated retinas. The images were taken at the nasal retina adjacent to the optic nerve. Scale bar, 100 μm. Mut, mutant.