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. 2012 Nov 14;288(2):915–926. doi: 10.1074/jbc.M112.411363

FIGURE 8.

FIGURE 8.

Rotenone treatment fails to induce mitophagy in Parkin−/− myocytes. A, representative images of WT and Parkin−/− myocytes. Cells infected with LC3-GFP were treated with DMSO or 40 μm rotenone for 1 h. After fixation, mitochondria were stained with anti-COXIV. B, quantitation of autophagosomes co-localizing with mitochondria. Mean ± S.E. (n = 3; *, p < 0.05 versus WT control, **, p > 0.05 versus Parkin−/− control). C, quantitation of the mean number of LC3-GFP positive autophagosomes per cell in WT and Parkin−/− myocytes. Mean ± S.E. (n = 3; *, p < 0.05 versus WT control, **, p > 0.05 versus Parkin−/− control). D, adult myocytes from WT or Parkin−/− mice were infected with β-Gal or Parkin for 24 h prior to 4 h of hypoxia and quantitation of cell death. Mean ± S.E. (n = 3, *, p < 0.05 versus Normoxia, **, p < 0.05 versus hypoxia).