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Roles of Ams2 destruction in meiosis. Meiosis was induced by thermal inactivation of Pat1 (pat1-114), and meiotic progression was monitored in WT myc-ams2+ (HYY952) and myc-ams2-dKm (HYY953) diploid cells. The samples were examined microscopically for the number of nuclei in a cell to monitor progression through meiosis (A) and analyzed by immunoblotting using specific antibodies every 20 min (B).
