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Ams2-dKm stimulates histone gene regulation in meiosis. A, Northern blot analysis of pat1-114-induced synchronized meiosis in WT myc-ams2+ (HYY952) and myc-ams2-dKm (HYY953) diploid cells. Total RNAs were isolated from samples in Fig. 4, and RNA levels of ams2+, mei4+, histone H2A+, H3+, and H4+ were examined. EtBr acts as a loading control. B, percentage of cells in the S phase (above) was estimated using FlowJo software and the Watson Pragmatic.
