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. 2012 Nov 29;288(2):928–937. doi: 10.1074/jbc.M112.410241

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FIGURE 6. — A feedback mechanism of Ams2 levels in G₁. A, WT myc-ams2⁺ (HYY908) and myc-ams2-dKm (HYY909) cells were grown to mid-log phase in YE+5S medium, and hydroxyurea was added to 20 mm final concentration. The samples were taken every hour for analysis by immunoblotting with indicated antibodies. B–D, same as A, but pof3Δmyc-ams2⁺ (HYY1094) and pof3Δmyc-ams2-dKm (HYY1067) (B), nrm1Δmyc-ams2⁺ (HYY1117) and nrm1Δmyc-ams2-dKm (HYY1119) (C), or cds1Δ myc-ams2-dKm (HYY1083) and chk1Δ myc-ams2-dKm (HYY1080) (D) cells were analyzed. E, asynchronous WT ams2⁺ (HYY908), ams2-dKm (HYY909), hst4Δ (HYY1032), and hst4Δams2-dKm (HYY1051) cells were spotted with decreasing cell number onto rich medium containing indicated DNA-damaging agents and incubated for 4 days.