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. 2012 Nov 26;288(2):938–946. doi: 10.1074/jbc.M112.424416

FIGURE 2.

FIGURE 2.

LGP2 basal ATP hydrolysis, but not dsRNA-stimulated ATP hydrolysis, is required for enhanced dsRNA recognition. A, diagram representing the domain structure of LGP2 and illustrating mutations. B, rate of ATP hydrolysis by LGP2 and mutants in the absence (black bars) or presence of 5 μg/ml of poly(I:C) (white bars). All mutants lack basal ATP hydrolysis activity, however, the presence of poly(I:C) stimulates LGP2 MIIa and K651E to hydrolyze ATP near wild type levels. * = p < 0.003; ** = p < 0.0001. C, analysis of ATP-enhanced RNA recognition by LGP2 and mutants, represented as the percent increase in the number of dsRNA molecules with binding events after addition of 500 μm ATP, compared with protein without ATP added. LGP2 displays an average 30% increase in the number of molecules bound with the addition of ATP, but no mutants display increased recognition in the presence of ATP. * = p < 0.003.