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. 2012 Nov 30;288(2):974–983. doi: 10.1074/jbc.M112.422295

FIGURE 5.

FIGURE 5.

Effect of Pak2 on MAPK activity. A, IgE-primed Pak2fl/fl and Pak2−/− BMMCs were stimulated with 100 ng/ml DNP-HSA for 0, 2, 5, and 10 min, and lysates were cleared and immunoblotted for anti-phospho-p38 (Thr-180/182), and anti-total p38. A representative blot is shown above, and below are shown the average of three independent experiments and standard deviations for relative -fold induction over wild-type cells for phospho-p38 (p-p38)/total p38. (*, p value <0.05, Student's t test) B, degranulation of IgE-primed Pak2fl/fl and Pak2−/− BMMCs stimulated with 30 ng/ml DNP-HSA antigen and incubated with p38 inhibitor SB203580 (10 μm) 30 min prior to assay. Average -fold change of three independent experiments and standard deviations (*, p value < 0.05, Student's t test) are shown. C, ERK1/2 was probed on immunoblots from Pak2fl/fl and Pak2−/− stimulated for 10 min with 100 ng/ml DNP-HSA antigen. A representative blot is shown, run for three independent experiments. pERK, phospho-ERK.