FIGURE 1.

Chondroadherin binding to heparin. Recombinant chondroadherin (30 μg) expressed in EBNA cells and the hbd-CKFPTKRSKKAGRH³⁵⁹ (30 μg), respectively, were passed over a heparin-Sepharose column (Amersham Biosciences). The column (1 ml) was washed with 5 mm sodium phosphate, 0.075 m NaCl, pH 7.4, and eluted with a gradient of 0.075–1 m NaCl (100% B) in 5 mm sodium phosphate, pH 7.4. Recombinant chondroadherin eluted with the peak at 0.6 m NaCl, whereas the peptide showed higher affinity eluting at 0.8 m NaCl (inset). The SDS-PAGE shows the preparation of chondroadherin applied to the heparin column (inset).