FIGURE 6.

SAXS analyses of FnI–II, FnI–II^Kt, and FnI-II^Kt + YbxF. (A) R_g in the absence and presence of saturating glycine in 5 mM and 10 mM Mg²⁺. (B) Kratky and (C) P(r) plots from data collected at 5 mM Mg²⁺ demonstrate glycine-binding-induced compaction. (D) Kratky plots comparing the effect of Mg²⁺, YbxF, and glycine on folding of FnI–II^Kt (in 5 mM Mg²⁺, except where noted). (E) Comparison of the experimental X-ray scattering profile for FnI–II^Kt with the scattering profile calculated (Materials and Methods) from the crystal structure (Butler et al. 2011) of FnI–II.