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Lariat species also identified by CircleSeq. (A) Nine read sequences including at least 30 nt from the splice donor of intron 2 of GAPDH also include sequence 5′ of the proposed branch point. (B) Intron 2 of GAPDH demonstrates enriched coverage after RNase R treatment, but no backsplice reads are detected. Plots of mapped read depth in untreated (green) and RNase R-treated (orange) samples shown at differing scales. (C) Expected effects of RNase R treatment on lariat structures.
