FIGURE 2.
Temperature-sensitive strains have additional m5C on tRNAHis when grown at nonpermissive temperature. (A) Assessment of viable cell titer following growth at 37°C. (B) m5C levels in tRNAHis and tRNAVal(AAC) during growth at 37°C. tRNAHis and tRNAVal(AAC) were purified from BY4741, thg1-Y146H, and fcp1-1 strains, and modification levels were measured by HPLC. m5C levels for tRNAHis (black) and tRNAVal(AAC) (gray) during temperature-sensitive strain growth at 37°C were plotted. m5C values during growth at 24°C were plotted as the 37°C 0-h time point.