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. 2012 Dec 18;12:296. doi: 10.1186/1471-2180-12-296

Figure 2.

Figure 2

YitA and YipA are only detectable in Y. pestis isolated from fleas but over-production of YitR increases their synthesis in vitro. Lane 1, molecular weight ladder. Lane 2, Y. pestis KIM6+ isolated from infected fleas. Lane 3, KIM6+ΔyitA-yipB isolated from infected fleas. Lane 4, KIM6+ grown at 22°C in BHI. Lane 5, KIM6+ (pWKS130::yitR) grown at 22°C in BHI. Lane 6, KIM6+ (pCR-XL-TOPO::yitR) grown at 22°C in BHI. Lane 7, KIM6+ΔyitA-yipB (pCR-XL-TOPO::yitR) grown at 22°C in BHI. Lanes 8–9, recombinant YitA and YipA purified from E. coli. Panels show Western blots probed with anti-YitA, anti-YipA, or anti-Ail (sample loading control) antiserum.