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Characterization of post-translational processing of YipA. KIM6+ (pCR-XL-TOPO::yitR) with the C-terminus of YitA (Lane 2) or YipA (Lane 4) tagged with mature β-lactamase were grown overnight at 22°C in BHI broth. KIM6+ YitA-β-lactamase (Lane 3) or YipA-β-lactamase (Lane 5) not over-producing YitR grown overnight at 22°C in BHI broth are provided as negative controls. Panels show Western blots probed with A) anti-YitA, B) anti-YipA, or C) anti- β-lactamase antiserum
