Table 2.
Y. pestis strains and plasmids used in this study
| Strain or Plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| KIM6+ |
Tc genes present, pCD1-negative |
[33] |
| KIM6+ΔyitR |
Deletion of the regulator YitR |
[9] |
| KIM6+ΔyitA-yipB |
Deletion of the Tc locus from YitA to YipB, YitR still present |
This study |
| KIM6+ (pCR-XL-TOPO::yitR) |
KIM6+ transformed with a high-copy plasmid producing YitR (Kan, Zeo) |
This study |
| KIM6+ΔyitA-yipB (pCR-XL-TOPO::yitR) |
KIM6+ΔyitA-yipB transformed with a high-copy plasmid producing YitR (Kan, Zeo) |
This study |
| KIM6+ (pWKS130::yitR) |
KIM6+ transformed with a low-copy plasmid producing YitR (Kan) |
This study |
| KIM6+ΔyitA-yipB (pWKS130::yitR) |
KIM6+ΔyitA-yipB transformed with a low-copy plasmid producing YitR (Kan) |
This study |
| KIM6+ YitA-β-lactamase |
YitA::β-lactamase translational fusion |
This study |
| KIM6+ YipA-β-lactamase |
YipA::β-lactamase translational fusion |
This study |
| KIM6+ YitA-β-lactamase (pCR-XL-TOPO::yitR) |
KIM6+ YitA-β-lactamase transformed with a high-copy plasmid producing YitR (Kan, Zeo) |
This study |
| KIM6+ YipA-β-lactamase (pCR-XL-TOPO::yitR) |
KIM6+ YipA-β-lactamase transformed with a high-copy plasmid producing YitR (Kan, Zeo) |
This study |
| KIM6+ (pCR-XL-TOPO::yitR) (pAcGFP1) |
KIM6+ (pCR-XL-TOPO::yitR) transformed with a plasmid producing GFP (Kan, Zeo, Amp) |
This study |
| KIM6+ΔyitA-yipB (pCR-XL-TOPO::yitR) (pAcGFP1) |
KIM6+ΔyitA-yipB (pCR-XL-TOPO::yitR) transformed with a plasmid producing GFP (Kan, Zeo, Amp) |
This study |
| Plasmids | ||
| pCR-XL-TOPO::yitR |
yitR expressed under native promoter from a high-copy plasmid (Kan, Zeo) |
This study |
| pWKS130::yitR |
yitR expressed under native promoter from a low-copy plasmid (Kan) |
[9] |
| pAcGFP1 |
Constitutively producing GFP (Amp) |
Clontech |
| pUC19 |
Cloning plasmid for creation of YitA- and YipA-β-lactamase fusion constructs (Amp) |
[34] |
| pDS132::yitA-β-lactamase |
Suicide plasmid containing YitA-β-lactamase fusion construct (Cam) |
This study |
| pDS132::yipA-β-lactamase |
Suicide plasmid containing YipA-β-lactamase fusion construct (Cam) |
This study |
| pET300/NT-DEST::yitA |
Production of YitA with an N-terminal His tag (Amp) |
This study |
| pET300/NT-DEST::yipA | Production of YipA with an N-terminal His tag (Amp) | This study |
a antibiotic resistances where present are noted in parentheses: Kan = Kanamycin, Amp = Ampicillin, Cam = Chloramphenicol, Zeo = Zeocin.