Table 3.

Primers used for yitR amplification, creation of KIM6+ΔyitA-yipB, and creation of YitA and YipA-β-lactamase fusion proteins

Primer Designation	Sequencea
YitR Forward Primer	5′-AGTTGAGCTCGTCTGCATTGATTATTTGACC-3′
YitR Reverse Primer	5′-AGTTTCTAGAGATCGTTGCGTAGCTGTGTTGC-3′
YitA-YipB KO Primer Forward	5′-TGGCATCAATAAACTGGCCTTTTCTGTTGCAC
	CAAAAATATGTGTAGGCTGGAGCTGCT-3′
YitA-YipB KO Primer Reverse	5′-TTCCCTATTCAAAATAGGGAAGGTGTTTAAAA
	TTAATAAACATATGAATATCCTCCTTA-3′
YitA C-terminus Forward	5′-ATCTCTAGACCCCAGAACACCCCATGTAT-3′
YitA C-terminus Reverse	5′-GATCTCGAGAAGATTAACTCTTAGCTTGT-3′
YitA Downstream Forward	5′-ATCCTCGAGGTCGACAAAAACGTTATTACATCCAA-3′
YitA Downstream Reverse	5′-GATGCATGCCTGATTTGACTGATTTTTCC-3′
YipA C-terminus Forward	5′-ATCTCTAGAAGAGACTTAAAGCATATGGT-3′
YipA C-terminus Reverse	5′-GATCTCGAGTCGATTCTGTTTGCTATATA-3′
YipA Downstream Forward	5′-ATCCTCGAGGTCGACTTAAGAATATTAAGGAGCCA-3′
YipA Downstream Reverse	5′-GATGCATGCTGGCCGTTCAGGTTGCAGTT-3′
Mature β-lactamase Forward	5′-ATCCTCGAGCACCCAGAAACGCTGGTGA-3′
Mature β-lactamase Reverse	5′-GATGTCGACTTACCAATGCTTAATCAGTGA-3′

a Underlined sequence denotes restriction enzyme site addition.