Table 1.
Characteristics of the real-time PCR assays used in the study
| Target | Species | Sequence | ACC No. | |
|---|---|---|---|---|
| ob |
Rat |
forward |
5′-CCAAAACCCTCATCAAGACC-3′ |
MN013076 |
| |
|
reverse |
5′-GTCCAACTGTTGAAGAATGTCCC-3′ |
|
| ob-Ra |
Rat |
forward |
5′-ACACTGTTAATTTCACACCAGAG-3′ |
D85557 |
| |
|
reverse |
5′-AGTCATTCAAACCATAGTTTAGG-3′ |
|
| ob-Rb |
Rat |
forward |
5′-TCTTCTGGAGCCTGAACCCATTTC-3′ |
D85558 |
| |
|
reverse |
5′-TTCTCACCAGAGGTCCCTAAACT-3′ |
|
| GAPDH |
Rat |
forward |
5′-AACGACCCCTTCATTGAC-3′ |
MN017008 |
| reverse | 5′-TCCACGACATACTCAGCAC-3′ | |||
Ob: leptin, ob-Ra –Rb; leptin receptor isoforms.