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PCR analysis of large and small pollen grains from p5cs1 p5cs2/P5CS2 plants. DNA extracted from pools of large and small pollen grains from p5cs1 p5cs2/P5CS2 plants was genotyped by PCR for the presence or absence of insertional mutations in P5CS1 and P5CS2. No DNA could be amplified from the small pollen population (bottom panel), while both p5cs1 and p5cs2 mutant alleles, as well as the wild type P5CS2 allele, were amplified from large pollens (top panel).
