Figure 2.
Surek-mediated killing of GD2-positive B78-D14 melanoma cells by mouse immune effector cells. Tumor cell killing was measured by an XTT-based long-term cytotoxicity assay using enriched T cells and peritoneal macrophages at an effector to target ratio of 50:1. Surek antibody mediated efficient killing of B78-D14 cells with a mean EC50 of 70 ng/ml [0.47 nM]. Anti-GD2 parental antibody Me361 was only effective at the highest tested concentration of 10,000 ng/ml; the addition of the second parental antibody 17A2 (anti-mouse CD3) did not improve killing activity, nor had the control trAb TRBs01 (anti-HER2/neu x anti-mCD3) any effect. The figure summarizes three independent experiments. Error bars indicate SD.