Figure 2.

2,930 PASA transcripts did not overlap with any of the currently annotated (ToxoDB) type II (ME49) Toxoplasma genes. Shown is a PASA transcript S2826 (Black) that aligned to a genomic sequence on TGME49_chrIV but did not overlap with any of the ME49 genes. We also show RNA-seq reads pile-up (histogram represented as reads per kilobase per million reads or RPKM) on the exons and 13 reads mapping to the exon-exon junction. This transcript produces an ORF of 146 amino acids, which is homologous to the TGGT1_124090 protein from the type 1 GT1 strain, but has no homology to any annotated ME49 protein.