Figure 5.

The ability to detect alternatively spliced isoforms using RNA-seq data is dependent on read coverage. Shown are fraction of alternatively spliced (from a total of 50) (Black bars) and multi-exonic genes (from a total of 5,873)(Grey bars) identified in bins (A) grouped by the Log₁₀ of the expression level as reads per kilobase (RPK) or (B) grouped by the Log₁₀ of raw coverage. Alternative isoforms are more likely to be detected amongst highly expressed genes compared to genes with low expression. See also additional file 9 for the relationship between read coverage and full assembly of transcripts.