Figure 2. Increased H3K27me3 in Dnmt^TKO ES cells. a, b,

Average normalized fold enrichment of ChIP-seq reads in 100 bp bins in Dnmt^TKO cells relative to wildtype for two representative loci, Tnp1 (a) and Prss21 (b). ChIP-seq peaks are indicated by the grey bars and genes are indicated at the bottom. c, qPCR validation of ChIP-seq peaks. Each bar represents the average percent input immunoprecipitated for six biological replicate ChIP experiments using chromatin from wild type v6.5 cells or Dnmt^TKO. Note that all six ChIP experiments are independent of those used to generate the ChIP-seq libraries. Primers used in (c) are listed in Supplementary Table 4 (* p<.05, ** p<.01, *** p<.001). d, Western blot of acid-extracted histones from v6.5 (+ or - 5-AzaC) and Dnmt^TKO cells. H1 is used as a loading control. Relative intensity of anti-H3K27me3 bands is shown on the bottom. Quantification was done using imageJ and normalized to H1.