Figure 5.

Effect of DHT on liver ApoM mNRA of DHT-treated C57BL/6 J mice. C57BL/6 J female mice were ovariectomized at the age of 3 months and treated at the age of 7 months. Animals were randomized into four groups (n = 6), with two groups receiving vehicle (propylene glycol) alone, and two groups receiving 3 mg/kg DHT. All animals were treated daily by sc injections for 7 or 14 d, fasted overnight, and killed using CO2. Livers were frozen in liquid nitrogen for ApoM RNA analysis. The levels of ApoM mRNA were determined by RT-PCR as described in “Materials and methods.” Data are expressed relative to the vehicle-treated group (100%). Data are represented as means ± S.D. (n = 6 for each treated group). Significant differences were determined using ANOVA. *P < 0.05 vs. vehicle-treated mice, ***, P < 0.01 vs. vehicle-treated mice.