Figure 4. Flux analyses of in vitro and in vivo experiments.
Graphs A and B represent the in vitro flux analysis of steroid hormones conversion at 48 h after addition of 200 nM A into the medium, without or with FSH 20 ng/ml. Graphs C, D, and E illustrate the in vivo flux analysis of steroid hormones conversion at several times of the estrus cycle (corresponding to diestrus, proestrus, and estrus stages). The aromatization reaction of A into E1 is taken as the reference reaction for each condition. The flux values for that reference were 7.29×10−9 pmoles/min/cell in vitro without FSH, 8.72×10−8 pmoles/min/cell in vitro with FSH, 6.09×10−9 pmoles/min/cell in vivo in the diestrus stage, 6.17×10−9 pmoles/min/cell in the proestrus stage, and 5.10×10−9 pmoles/min/cell in the estrus stage of the estrous cycle. Values for the other reactions in each condition are relative to the corresponding reference. Arrow thicknesses are proportional to the flux absolute values.