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. 2013 Jan 11;8(1):e54173. doi: 10.1371/journal.pone.0054173

Figure 6. Upregulation of Ghrelin expression in CPTD6 islets.

Figure 6

Immunohistochemistry for ghrelin (B, E, H, K, N, Q) was performed in combination with insulin (A, D), glucagon (G, J), and somatostatin (M, P). Merged images with DAPI nuclear counter-staining are shown (C, F, I, L, O, R) on pancreata from control (A, B, C, G, H, I, M, N, O) and CPTD6 (D, E, F, J, K, L, P, Q, R) mice. Levels of insulin and glucagon expression are markedly reduced or lost in the majority of islet cells of CPTD6 mice in comparison with the controls, while the number of somatostatin positive cells appears more moderately reduced. Very few, if any, ghrelin positive cells can be found in the islets of control adult mice (B, C, H, I, N, O). In contrast, strong staining is observed in many cells of the CPTD6 pancreata, located predominantly around the periphery of the islets. Images are representative of at least four animals from each group. Scale bars, 50 µm. (S, T, U, V). Quantification of the relative numbers of hormone expressing cells in the islets of Langerhans of control and CPTD6 animals shown as the percentage of immunofluorescence positive cells per total number of DAPI positive cells. (S) Insulin producing cells (control 72% >19% CPTD6), (T) Glucagon producing cells (control 11.4% >5.3% CPTD6) (U) Somatostatin producing cells (control 11.6% >9.5% CPTD6), and (V) Ghrelin producing cells (control 0.6% >20.6% CPTD6).