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. 2012 Nov 21;10:229. doi: 10.1186/1479-5876-10-229

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Copyright ©2012 Mamidi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Differentiation studies: Multilineage differentiation potential of cultured BM-MSCs injected via different bore size needles along with controls. (A - D) Chondrogenic differentiation potential was demonstrated by Alcian blue staining. (E - H) Formation of mineralized matrix was detected by alizarin Red staining confirms the osteogenic differentiation. (I - L) Adipogenesis was confirmed by neutral oil droplet formation stained with Oil Red O. (M) Represents viability, stromal marker expression and mesodermal tri-lineage differentiation potential of MSCs ejected through 26 G needle after cryopreservation.