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. 2013 Jan 11;8(1):e53701. doi: 10.1371/journal.pone.0053701

Figure 5. Androgen Receptor-mediated repression of Sox2 expression can be reversed by treatment with the Anti-Androgen MDV3100.

Figure 5

A) To verify that the decrease of Sox2 protein is specific to AR activation and can be reversed by an AR antagonist, CWR-R1 cells were grown in 1 nm R1881 or vehicle for 24 hrs, and then either 10 µM MDV3100 or vehicle was added to the culture medium for an additional 48 hrs. Western blots show a decrease in Sox2 protein with R1881 that can be returned to basal levels with addition of MDV3100, without any change in AR protein. β-Actin was used as a loading control. A schematic outlines the time frame of these experiments. B) A decrease of Sox2 mRNA with R1881 treatment was measured using qPCR (*p<0.05), which was brought back to control levels upon treatment with MDV3100. C) A time course of treatment with a known inhibitor of transcription, 10 µM Actinomycin D, yielded a similar rapid decrease of Sox2 mRNA in CWR-R1 cells upon AR stimulation with R1881 as measured by qPCR. Levels at 0.25 hrs and beyond represent a statistically significant decrease in Sox2 mRNA (p<0.05), showing similar kinetics of transcriptional repression with both R1881 and Actinomycin D drug treatments.