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. 2013 Jan 11;8(1):e53737. doi: 10.1371/journal.pone.0053737

Figure 4. FRET-analysis of NF-κB subunits and IκB.

Figure 4

A) Fusion constructs that have been applied for FRET-measurements. B) The FRET-efficiency between the FRET-pairs p50-ECFP/RelA-EYFP, p50-ECFP/IκB-EYFP, RelA-ECFP/IκB-EYFP, p50-ECFP/Abi5-YFP, RelA-ECFP/Abi5-YFP and IκB-ECFP/Abi5-YFP was measured in more than 40 plant cells for each combination. The data represent mean average ± S.E. C) Crystal structure of NF-κB/IκB trimer and the distances between RelA-C-terminus and p50-N-terminus or IκB-C-terminus, respectively. The crystal structure was obtained from RCSB protein data bank, accession number 1IKN [31].